Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays
Summary
Spatially resolved transcriptomic technologies are promising tools to study complex biological processes such as mammalian embryogenesis. However, the imbalance between resolution, gene capture, and field of view of current methodologies precludes their systematic application to analyze relatively large and three-dimensional mid- and late-gestation embryos. Here, we combined DNA nanoball (DNB)-patterned arrays and in situ RNA capture to create spatial enhanced resolution omics-sequencing (Stereo-seq). We applied Stereo-seq to generate the mouse organogenesis spatiotemporal transcriptomic atlas (MOSTA), which maps with singlecell resolution and high sensitivity the kinetics and directionality of transcriptional variation during mouse organogenesis. We used this information to gain insight into the molecular basis of spatial cell heterogeneity and cell fate specification in developing tissues such as the dorsal midbrain. Our panoramic atlas will facilitate in-depth investigation of longstanding questions concerning normal and abnormal mammalian development.
PA53 Scan Series
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PA53 FS6 Scan, this scanning platform provides full flexibility on scan and stitch of different fluorescent sample. We can select the use of HBO illuminator or high quality LED illuminator, using combination of different fluorochromes; with or without extended focus (excellent solution for FISH), or z-stacked |
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PA53 Bio Scan mainly meant for transmitted light scan. Various contrasting method can be used for scan and stitch, from Phase contrast to polarizing contrast…… Scanning of transparent sample is not a problem now. |